Hyperexpression of some non invasive markers have been studied previously in setting of acute rejection in renal allograft recipients and necessitated to confirm the observation in larger sample size. We at our institute isolate post transplant and at time of graft dysfunction. Clinical setting includes acute rejections, UTIs, CMV, CAN and DGF. Between July 2007– Feb 2008, 135 samples from 50 consecutive transplant recipients processed for above mentioned genes. We noticed high level expression of cytokines from both cytotoxic and regulatory T cells on first day post transplant, which showed declining pattern of these genes on day 7 and 3 months post transplant. We were also able to demonstrate again rising expression of GB, Perforin and FOX P3 with acute rejections. Results of these findings will be analysed and presented. gene Day 1 post tx Day 7 post tx 3 months post tx GB (RNA copy no. median) 47000 11200 4400 Perforin (RNA copy no. median) 110000 24500 8200 FOX P3 (RNA copy no. median) 77000 44000 00 Conclusion: The findings of our study are of importance when designing studies using the cytokine gene expression profile as a marker for graft dysfunction in setting of renal transplantation.
Volume : 6
Issue : 4
Pages : 3
Sindh Institute of Urology and Transplantation (SIUT), Karachi, Pakistan.