To our knowledge, there are no studies evaluating the influence of the kidney’s weight before and after washing on the outcome of the transplantation. As a result, the present study was designed to evaluate the impact of weight changes following kidney wash on the post transplantation creatinine levels. A total 92 renal transplantations from living donors performed between March 2007 and 2008, in Sina Hospital were evaluated. The patients who experienced rejection or died during the study period were excluded. Demographic, anthropometric data along with laboratory data from donors and recipients were collected in the pre-operative phase. Data about kidney weight both before and after washing were obtained using an electronic digital weighing machine immediately after the donor's nephrectomy. The recipients were followed for 6 to 12 months and the data on their body weight, serum creatinine levels and the development of CMV infection. During the study period 15 patients were excluded. Both donors and recipients were predominantly males (donor: 80%, recipients: 65%). There was no significant difference between the age, weight and height of recipients and donors. Mean kidney weight before and after washing was 157.5 ± 31.3 and 180.8 ± 33.7 kg, respectively. Warm and cold ischemic time were 4.3 ± 1.5 and 49.8 ± 15.5 minutes, respectively. There was no significant relation between the recipient creatinine level after 6 and 12 months and the donor's gender, age, weight and height. The kidney's weight after washing and the cold ischemic time was related to the donor's weight (p value<0.001, 0.027, respectively). In conclusion, the post transplantation creatinine level is not influenced by either of the donor or recipient demographic characteristic nor the kidney’s weight before or after the washing process.