During cold preservation of liver grafts, expression of tissue adhesion molecules has been reported, as a factor that is indicative of preservation injury. Some biochemical agents as well as increased levels of intracellular calcium, also play important roles in preservation injury during cold storage. In the current study, we aimed to test if the addition of a calcium channel blockers, Sodium nitroprusside, or glutathione into preservation solution, would reduce upregulation of adhesion molecules thus leading decreased preservation injury in rat liver.Fifty albino Wistar rats, weighing 200 ± 50 g, were divided into 1 control (perfused with Wisconsin solution, without preservation) and 4 study groups of rat livers (ten livers each). Study groups livers were harvested, perfused, and preserved for 16 hours in 4 different solutions (Wisconsin solution alone, Wisconsin solution+verapamil, Wisconsin solution+NaNitroprusside and Wisconsin Solution+ glutathione). At the end of the preservation time, levels of graft tissue adhesion molecule (ICAM-1) expression, were analyzed.Sixteen hours’ preservation with Wisconsin solution alone and Wisconsin solution+ verapamil perfusates caused significantly more ICAM-1 expression than did 16 hours’ preservation with Wisconsin solution+Nanitroprusside and Wisconsin solution+glutathione perfusates (P=0.010). No significant difference was found for ICAM-1 expression between the Wisconsin solution+Nanitroprusside and Wisconsin solution+glutathione groups. Minimal ICAM-1 expression was demonstrated in the control group (P=0.0003). Addition of sodium nitroprusside and glutathione into the Wisconsin solution decreased levels of ICAM-1 molecule expression, which reflects lower levels of preservation injury. In this study, verapamil added to the perfusate/preservation solution for reducing the intracellular calcium accumulation, had no effect on tissue ICAM-1 molecule expression