Organ donation is a cultural activity. Years of education and cultural development are required to increase organ donators and hence enhance organ transplantation. In the way of efficient development and implementation of educational and cultural modalities, an especial attention should be paid to the characteristics of the target groups. In our study, we report the characteristics of organ donation volunteers in our country. Using the organ donation database at Organ procurement unit of Masih hospital, Tehran, Iran, we used the registration data from 2005 to September 2008. Demographic data about organ donation volunteers’ database was extracted. A total of 182877 volunteers were registered at our database. 10025 (54.7%) of the volunteers were female. 2.2% were under 18 years old, 20% were between 18 to 25 years old and 77.8% were above 25 years old. Regarding their education, 8.64% were under high school diploma, 36.8% had high school diploma and 53.7% had bachelor or higher degree. Thirty percent of volunteer were introduced by their friends and relatives and 25% got familiar with the unit through the media. In conclusion, in Iran, people with a higher education are most likely to donate an organ. Also, the role of person-to-person contact was more prominent than the media. Reaching out to people with no or a lower academic degree and a stronger role of the media may cause an increase in the number of organ donation volunteers.