Despite the increasing number of transplantation in Iran, organ shortage and long waiting list are major problems in the country. Many publications demonstrated that the healthcare professionals’ willingness to participate in donation process can improve donation rate. Among the health care staff, nurses are usually the first people who recognize the patient as a potential donor. So they can have an important role in procurement of organs and tissues from deceased donors. Our objectives were a survey of nurses’ knowledge and attitudes toward Organ and Tissue Donation and examining the effect of an “organ donation workshop” on the nurses. A 39-item questionnaire was completed by 66 nurses, before and after participation in a 1-day “organ donation workshop” that was held at Iranian Tissue Bank (Tehran-Iran). The questionnaire contained demographic data, 29 questions regarding knowledge and 8 questions for attitudes toward organ and tissue donation. 69.7% of participants were women and 30.3% were men. The mean score for knowledge was 16.89 (SD= 3.33) before and 23.76 (SD= 1.66) after workshop (P=0.0001). The mean attitudes score was 4.76 (SD=1.71) before and 5.08 (SD=1.34) after workshop (P=0.235). Although 69.7% claimed they were willing to have a donation card, but only 19% of them actually carried it. This study showed that educational programs should enhance nurses’ knowledge and commitment to organ donation process and finally increase the donation rate. So, this is of great importance that organ procurement units focus on regular training programs for all healthcare staff.