Development of organ transplantation is linked with organ procurement. In many cases, such organs should be harvested from brain dead patients. Failing in such a task is the major cause of mortality in the waiting list of organ transplantation all over the world. In many cases, failing to acknowledge the existence of such patients and proper declaration to transplantation centers or other responsible authorities are the causes. To overcome this problem, organ procurement unit was developed in 2004 in Masih Daneshvari Hospital, Tehran, Iran. It is a university-based third-level hospital and a referral center for lung transplantation from all over the country. The unit covers some private and public hospitals in Tehran and some other cities. To unify the procedures, nursing coordinators of the covered hospitals participate in regular meetings and educational classes and then contacted daily about new cases of brain death. Representatives from organ procurement unit pay regular onsite visits to hospitals to assure quality of the procedure and provide education and assistance to physicians and nurses. When organ procurement unit is contacted by hospital authorities about a case of brain death, the unit sends a trained and experienced representative to obtain electroencephalogram (EEG) and confirm the brain death. After that, if the patient’s family were willing to donate the organs, patient is transferred with equipped ambulances to Masih Daneshvari Hospital. During this time the transplantation management center of the ministry of health is informed about the patient. The center coordinates and allocates organs between transplantation centers in the country. In our hospital, after admission to ICU, the final and legal approval of brain death by four different specialists from the ministry of health takes place and the family members of the patient sign the final consent for organ donation. After coordination with transplantation teams around the country, the harvest procedure takes place and the organs are sent to destination transplantation centers. The final step is to pay tribute to patient by attending the funeral by representatives from organ procurement unit. The described system assures quality care to brain dead patient along with proper and legal organ allocation. It could be used as a model for development of other organ procurement units in the region.