The aim of this study is to evaluation of safety and complication rate of one knot one suture technique in the transplantation. Between May 2006 to June 2008 four hundred renal transplantation were done in the Labbafinejad hospital, Shaheed Beheshti University of Medical Science, Tehran, Iran and 150 patients roll into the study randomizely. In these patients demographic data, anastomosis time, systolic and diastolic pressure, number of graft artery, anastomosis sites, serum creatinine, complications and follow up time were analyzed retrospectively. Mean age was 37 years (6 – 71). 97 patients were men (%67.7) and 53 patients were women (%35.3). Mean follow up was 18.5 months (4 -26). Arterial anastomosis time varied between 6-9 minutes (mean 7) and vein anastomosis time varied between 7-10 minutes (mean 8). Blood systolic pressure vary between 90 – 170 mmHg (mean 130 mmHg) and diastolic pressure vary between 50 -110(mean 75mmHg).144 graft has one artery (%96) and remain has two arteries(%4).Sites of artery anastomosis were: common iliac in 77 (%47/5), internal iliac in 66 (%44),external iliac in 8 (%5/3), aorta in 4 (%2/7) and inferior mesenteric artery in 1 (%0.7). Vein anastomosis sites included external iliac in 146 (%97/4), common iliac in2 (%1.3) and inferior vena cava in 2 (%1.3). Number of the first ,second and third transplantations were 146 (%97.3), 3 (%2) and 1 (%0.7) respectively. Rate of serum creatinine level vary between 0.5 – 3.5 mg/dl (mean 1.5 mg /dl). There isn’t any vascular complications in follow up period .Rejection occur in 4 (%2.7) and one patient return to dialysis. In conclusion, One Knot One Suture is a safe, rapid and with the low complication rate Technique