Management of live donor is very important for future of donor. There have been approximately 60,000 living donor nephrectomies in the world to date, and approximately 20 known deaths due to the operation. The most common cause of death has been pulmonary embolus. Reported incidence of complications includes: Pancreatic injury: 0.2% Splenic injury: 0.3%, Arterial complication of arteriogram: 0.5%, Peripheral nerve palsy: 1.1%, Deep venous thrombosis: 1.9%, Hernia: 2.0%, Wound infection: 2.1% Pneumonia or pleural effusion: 4.3%, Urinary tract infection: 8.6%, Pneumothorax: 9.1%, Atelectasis: 13.5%, Hypertension (late): 15%. Post operation Wound infection is the third most common nosocomial infection, which dependent to surgeon and his or her team, theater room, number and virulence of contaminated bacteria, patient (immunity and defense), and time of administration and duration of antibiotic. Since 1960 there is some guideline for prophylaxis of wound infection especially for antibiotic. Some centre treat wound as infection instead of prophylaxis for infection. Approach of prophylaxis for wound infection in donors is different between transplant centers, in our center, donor takes antibiotic conventional at least seven days post discharge from hospital. We had trial case study about antibiotic prophylaxis in live donor for changing conventional approach. Hundred kidney donors classified randomly in two group: group one fifty kidney donors, in them according our conventional method immediately post operation antibiotic is given for at least seven days. Group two, fifty kidney donors, in them one gram cephazoline IV injected before anesthesia and continued for 24 hours. And surgical wound followed for one month in both groups. In group one one case and in group two two case had secretion p value =0.5 diferrence was not significant. Antibiotic prophylaxis starting before incision and continued for 24 hours in donor nephrectomy is safe and effective and cost benefit in preventing wound infection.