Laparoscopic donor nephrectomy is becoming the standard of care in many transplant centers. In this study we present our experience and evaluate the outcome of donors and recipients done in our center. Between March 2003 and August 2006, 400 cases of laparoscopic donor nephrectomy were performed in our institution. Donors were evaluated as regards renal vasculature using CT renal angiography. We used the left kidney in 329 patients and the right kidney in 71 cases. All cases were performed trough transperitoneal route. We used three trocars on the left side and 4 trocars on the right side to perform the surgery. Kidney was extracted manually through a 7 cm Pfennestiel incision. All cases were completed laparoscopically with no open conversion. The mean operative time was 117±34 min. The mean blood loss was 56±28 cc, and none of the donor required blood transfusion. The mean warm ischemia time was 2.6±0.4 min. The mean renal artery length was 3.1±0.4 cm. the mean renal vein length was 2.4±1.2cm. The mean ureter length was 14.3±2.1 cm. Donors were discharged on second postoperative day. None of the donors required readmission. Kidneys were transplanted successfully and mean creatinine of the recipient on discharge was 1.2±0.6 mg/dl. One patient had renal artery thrombosis on post-operative day 2. One other patient with double renal arteries had thrombosis of the smaller artery just after the anastomosis. Seventeen patients had ATN and four of then required dialysis. Kidney function recovered thereafter in all of them. Laparoscopic surgery is a minimally invasive approach for live donor nephrectomy with good functional outcome. Donor benefits from lesser morbidity without compromising the anatomical or physiological outcome of the nephrectomized kidney.