For the last eleven years, Hospital de la Santa Creu i Sant Pau (HSCSP) has been running an educational program on organ and tissue transplantation for high school students. The main goal is to promote organ and tissue donation. The program is designed in four parts: a talk by a transplant coordinator on general concepts, a talk by a nephrologist comparing dialysis-versus-kidney transplantation, interviews with already-transplanted kidney patients, and interviews with waiting-list patients while undergoing dialysis. The objective of this work was to evaluate the impact of this program on students. A total of 465 students who participated in the 2005/2006 sessions were asked to fill in a 30-question survey before attending the talks in order to evaluate their knowledge and attitude regarding donation. The students were invited to answer the same survey 6 months later. The two surveys were then compared to evaluate the impact of the talks. The talks were useful in conveying knowledge about the severe shortage of organs and the role of transplantation as the only viable option for many diseases. A positive attitude to organ donation was promoted since only 5% of the students expressed any uncertainty or disagreement about donation 6 months after the talks. An educational program on organ transplantation is a good strategy to create awareness among high school students and to promote organ donation. Similar programs could be implemented worldwide in order to increase the organ pool.