In the present study we investigated whether ischemia/ reperfusion (I/R) of the intestine can induce translocation of bacteria, endotoxin, and pancreatic juice into the blood. The levels of amylase and lipase in the blood were measured to reflect the dislocation of the pancreatic juice. Plasma endotoxin levels were measured with the Limulus amebocyte lysate assay. Levels of E. coli DNA, as measured by polymerase chain reaction (PCR), reflected bacterial translocation. Reperfusion of the intestine induced a significant increase in the levels of endotoxin and pancreatic juice in the blood. However, there was no bacterial translocation into the blood. Organ injury parameters, such as the blood concentrations of ALT, LDH, CKMB, and Cr, and the lung weight/body weight ratio (LW/BW) were all increased significantly after ischemia/reperfusion (I/R) challenge. Inflammatory parameters such as WBC count and hydroxyl radical, nitric oxide, and tumor necrosis factor levels were also increased significantly. After administration of enzymes inhibitor, FOY (10mg/kg) the liver injury was significantly attenuated. These findings suggest that I/R of the intestine induce multiple organ injuries that appears to be more dependent on the translocation of pancreatic enzymes.