The aim of this study was to investigate the effect of rapamycin (RAPA) on the healing of the bladder and abdominal wound closure. This study was performed in research laboratories at our center. Fourteen male Sprague Dawley rats were randomized to receive either RAPA (3 mg/day) or placebo. A midline laparotomy was performed. The bladder was cut and closed with 4-0 Vicryl in a double layer. The fascia was closed with 0 nylon suture, and the skin closed with a subcuticular 2-0 nylon suture. The mean serum RAPA level was 9.1ng/mg. Eosinophil and neutrophil infiltration, and presence and the degree of myofibroblast were significantly high in the bladder, fascia, and dermis in the control group. Lymphocyte infiltration was similar in each group. Mean microvessel density, percentage of cells expressing vascular endothelial growth factor in the bladder, fascia, and dermis were significantly lower in the RAPA group. Both PCNA labeling indexes for inflammatory cells in the fascia, dermal fibroblast, and epithelial cells in the placebo group were significantly higher. No difference was observed for the hydroxyproline levels in both the bladder and fascia between the groups. We found that RAPA treatment affects all steps of the wound healing process by decreasing the inflammatory cell number, angiogenesis, and myofibroblast proliferation, so the wound healing process is delayed and consequently the tensile strength of the wound is decreased.