The plasma of transplanted patients induces increased rates of mononuclear cell apoptosis in spite of normalization of serum creatinine. Immunosuppressive medication may be one of the possible mechanisms of altered apoptotic pattern. We evaluated the in vitro effect of different doses of Cyclosporin, Mycophenolate and steroids on apoptosis in human cultured monocytic U937 cells. Apoptosis was measured by fluorescence microscopy and Annexin V assay. Increasing the concentration of cyclosporin (100 to 800 ng/mL) induced progressive increase in apoptosis rate (16 to 32%). Combination of steroid 0.01 mg/mL and cyclosporin increased apoptosis rate to 45%. Mycophenolate alone 0.3 mg/mL lead to an apoptosis rate of 34%. Therapeutic levels of mycophenolate between 3-7 mg/mL lead to apoptotic rate of 56%, 67% respectively. Triple combination of cyclosporin, steroid and mycophenolate increased apoptosis rate to 95%. Immunosuppressive therapy might contribute to the high rate of apoptosis observed in mononuclear cells of transplanted patients. This may affect patients susceptibility to infections and contribute to different drug mechanism of immunosuppression.