Episodes of acute rejection, is considered an important risk factor for the development of chronic allograft nephropathy. Different studies showed pre transplant cytokine profiles in recipients’ blood are associated with transplant outcome. A total of 57(38 male and 19 female, age 36±5 years) patients candidate for unrelated living kidney transplantation were selected for this investigation. Serum samples were collected 24 hours pre transplantation, one week and two week post transplantation and during every rejection. Immunosuppression consisted of a triple drug immunosuppressive regimen containing cyclosporine, prednisolone and mycophenolate mofetil. From the transplanted patients 19(33.3%) individuals showed an acute rejection episode based on increasing the serum creatinine and blood urea nitrogen during 14 days after transplantation. TGFa;, IL2 and IFNa; serum level were determined by an ELISA method using Bindermed system kits (Germany) The mean TGFa; concentration increased in the patients with acute rejection episode to those of stable graft without any sign of rejection, before transplantation (85394 vs. 75265 pg/ml P=0.34) and one week after transplantation (84390 vs. 77558 pg/ml). The mean concentration of IL2 increased significantly in patients with rejection before and 1 week after transplantation (15 vs6.8 pg/ml P=0.005 and 19 vs. 4.85 pg/ml P=0.001) and the mean concentration of IFN947; increased significantly before and after transplantation in patients with acute rejection (161.1 vs. 65.2 and 175.6 vs. 66.5 pg/ml P<0.001). In conclusion it is suggested that cytokine study especially Th1 cytokines before transplantation could be a valuable predictive marker for acute rejection episode.