Figure 2. Hypothermia of 4C had no significant influence on the percentage of porcine aortic endothelial cells stained positively with BS-I Isolectin B4. Not even a long storage time of 6 hours at 4C resulted in a decrease of α-Gal positive cells. Nearly all porcine aortic endothelial cells cooled at 4C, as well as those incubated at 38C showed Galα1-3Galβ1-4GlcNAc-Rs on their surface.