Figure 2. Peripheral lymphocyte subsets in kidney-transplant
recipients before vaccination according to their immunosuppressive treatment.
(A)Absolute counts of peripheralCD3+CD4+,CD3+CD8+,CD19+, andCD3-CD16+CD56+ cellswere obtainedwith TruCount tubes and the following combinations
ofmonoclonal antibodies: CD3-FITC/CD16+CD56-PE/CD45-PerCP/CD19-APC and CD3-FITC/CD8-PE /CD45-PerCP /CD4-APC. (B) Percentages of peripheral
CD19+ B-cells expressing CD5 or CD27 were analyzed with CellQuest software and the following combination of monoclonal antibodies: CD27-FITC/CD5-
PerCP/CD19-APC. (C) Percentages of unswitched (IgM+ IgD+) and switched (IgM- IgD-) among CD19+CD27+ memory B-cells were analyzed with CellQuest
software and the following combination ofmonoclonal antibodies: CD27-FITC/IgD-PE /CD19-PerCP/IgM-APC.
Healthy blood donors (white bars), patient group 1 (gray bars), and patient group 2 (hatched bars).
$Data aremissing for one patient fromgroup 1.
The data show themedian values and the 10th, 25th, 75th, and 90th percentiles.All values above the 90th or below the 10th percentile are detailed.