Figure 7. (A) Pro-apoptotic relative Bax/Bcl-2 mRNA expression in AAVCMV hVEGF-B overexpressing cardiac allografts vs control allografts. Results were normalized to GAPDH. (B) Real time RT-PCR results of selected metabolic genes in AAV-VEGF-B cardiac allografts at 6 hours after reperfusion. (c) Representative pictures from immunohistochemically stained AAV-hVEGF-B transduced cardiac allograft cross-section 6 hours after reperfusion, showing graft infiltrating ED-1-positive macrophages, MPOpositive neutrophils, CD161a-positive NK cells, OX-62-positive dendritic cells, and CD4-positive and CD8-positive T-cells. (D) Cardiac allografts were stained with Masson-Trichrome and cross-sections were scored semiquantitatively in a blinded manner for the extent of fibrosis. Scale bars in C and D = 5 μm. All measurements were performed from cardiac allografts 6 hours after reperfusion.