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Volume: 6 Issue: 4 November 2008 - Supplement - 1

FULL TEXT

STUDY OF INVASIVE ASPERGILLOSIS IN LIVER TRANSPLANT RECIPIENTS BY NESTED PCR

Invasive aspergillosis in liver transplant recipients still represents serious complications and is associated with a significant decrease in survival. Invasive aspergillosis occurs in 1 to 8% of liver transplant recipients and associated with high level mortality. Despite extensive investigation on methods such as serologic techniques to improve the rapid diagnosis of these infections, the diagnosis of invasive mycoses remains largely dependent on clinical presentations. The aim of this study was to describe the incidence and outcomes of invasive aspergillosis in liver transplant recipients by Nested PCR. From March 2004 to July 2008, 408 recipients underwent liver transplantations in solid organ transplant unit in Nemazi Hospital, Shiraz University of Medical Sciences, Iran. Sera from patients suspected to fungal infections were extracted for Aspergillus DNA. The PCR was performed as a nested PCR with two sets of primers. It is worth mentioning that this PCR is able to identify all Aspergillus species (panfungal aspergillosis). The lower limit of detection of this PCR assay was one colony forming unit/ml of serum. Of these 408 recipients, 42 patients were suspicious to invasive aspergillosis and Nested PCR was positive in 19 (4.6%) patients with proven & probable criteria. The mean time for the onset of invasive aspergillosis following transplantation was 44 days in the present study. In conclusion, this study has determined the incidence of Aspergillus infection after liver transplantations. Considering the above findings, preventive strategies should focus on reducing both environmental and host risk factors.



Volume : 6
Issue : 4
Pages : 198


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Professor Alborzi Iinfectious Disease Research Center,2. Shiraz Transplant Center, Namazi Hospital, and Transplantation Research Center, Shiraz University of Medical Science, Shiraz, Iran